This class will provide a demonstration of how to identify cell types based on statistics, visualization, and canonical markers. One Peripheral blood mononuclear cells (PBMCs) sample will be used to illustrate a basic Single Cell RNA-Seq analysis pipeline. Partek Flow is a web-based application for the analysis of next generation sequencing (NGS) including RNA, small RNA, and DNA sequencing. With an easy-to-use graphical interface and the ability to build custom analysis pipelines, Partek Flow enables users to carry out routine NGS data analysis using dozens of popular algorithms without writing codes or running command lines tools. Attendees will need to have taken the Partek Flow Basic Components class before registering or be comfortable with Partek Flow.
By the end of this class, attendees will be able describe how to access Partek Flow from the NIH Library, discuss the Quality Control (QC) and Quality Assurance (QA) tools, identify pre- and post-alignment tools, describe options for quantification and normalization, and perform pathway analysis and visualization.